analyse gene over-representation with GSEA — test_gene

test_gene_overrepresentation() takes as input a `tbl` (with at least three columns for sample, feature and transcript abundance) or `SummarizedExperiment` (more convenient if abstracted to tibble with library(tidySummarizedExperiment)) and returns a `tbl` with the GSEA statistics

test_gene_overrepresentation(
  .data,
  .entrez,
  .do_test,
  species,
  .sample = NULL,
  gene_sets = NULL,
  gene_set = NULL
)

# S4 method for class 'spec_tbl_df'
test_gene_overrepresentation(
  .data,
  .entrez,
  .do_test,
  species,
  .sample = NULL,
  gene_sets = NULL,
  gene_set = NULL
)

# S4 method for class 'tbl_df'
test_gene_overrepresentation(
  .data,
  .entrez,
  .do_test,
  species,
  .sample = NULL,
  gene_sets = NULL,
  gene_set = NULL
)

# S4 method for class 'tidybulk'
test_gene_overrepresentation(
  .data,
  .entrez,
  .do_test,
  species,
  .sample = NULL,
  gene_sets = NULL,
  gene_set = NULL
)

# S4 method for class 'SummarizedExperiment'
test_gene_overrepresentation(
  .data,
  .entrez,
  .do_test,
  species,
  .sample = NULL,
  gene_sets = NULL,
  gene_set = NULL
)

# S4 method for class 'RangedSummarizedExperiment'
test_gene_overrepresentation(
  .data,
  .entrez,
  .do_test,
  species,
  .sample = NULL,
  gene_sets = NULL,
  gene_set = NULL
)

Arguments

.data: A `tbl` (with at least three columns for sample, feature and transcript abundance) or `SummarizedExperiment` (more convenient if abstracted to tibble with library(tidySummarizedExperiment))
.entrez: The ENTREZ ID of the transcripts/genes
.do_test: A boolean column name symbol. It indicates the transcript to check
species: A character. For example, human or mouse. MSigDB uses the latin species names (e.g., \"Mus musculus\", \"Homo sapiens\")
.sample: The name of the sample column
gene_sets: A character vector. The subset of MSigDB datasets you want to test against (e.g. \"C2\"). If NULL all gene sets are used (suggested). This argument was added to avoid time overflow of the examples.
gene_set: DEPRECATED. Use gene_sets instead.

Value

A consistent object (to the input)

A `spec_tbl_df` object

A `tbl_df` object

A `tidybulk` object

A `SummarizedExperiment` object

A `RangedSummarizedExperiment` object

Details

`r lifecycle::badge("maturing")`

This wrapper execute gene enrichment analyses of the dataset using a list of transcripts and GSEA. This wrapper uses clusterProfiler (DOI: doi.org/10.1089/omi.2011.0118) on the back-end.

Undelying method: msigdbr::msigdbr(species = species) |> nest(data = -gs_cat) |> mutate(test = map( data, ~ clusterProfiler::enricher( my_entrez_rank, TERM2GENE=.x |> select(gs_name, entrez_gene), pvalueCutoff = 1 ) |> as_tibble() ))

Examples


print("Not run for build time.")
#> [1] "Not run for build time."

#se_mini = aggregate_duplicates(tidybulk::se_mini, .transcript = entrez)
#df_entrez = mutate(df_entrez, do_test = feature %in% c("TNFRSF4", "PLCH2", "PADI4", "PAX7"))

if (FALSE) { # \dontrun{
  test_gene_overrepresentation(
    df_entrez,
    .sample = sample,
    .entrez = entrez,
    .do_test = do_test,
    species="Homo sapiens",
   gene_sets =c("C2")
  )
} # }